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Ionic® Tissue to Pure DNA
Automated extraction from a variety of tissue types
VIEW BROCHURE REQUEST INFOThe Ionic® Tissue to Pure DNA Kit provides automated purification of DNA from fresh frozen tissue samples with less hands-on time than conventional bead and column-based methods
The Ionic Purification System from Purigen Biosystems uses isotachophoresis to extract, purify, and concentrate RNA and DNA from cells, FFPE, and fresh frozen tissue. Since nucleic acids remain in their native form, not denatured or dehydrated, the Ionic system is ideal for challenging samples with limited starting material or low-quality material.
Get pure, native DNA from fresh frozen tissue samples with higher yield
with only 5 minutes of total hands-on time per sample.
FIGURE 1: DNA extraction was performed on 8 different tissue types with four different extraction methods on a total of 632 specimens. Each tissue type/condition has an n-value ranging from 6 to 47 individual specimens. Pancreas, brain, breast, and heart specimens were derived from one donor and kidney, liver, lung, spleen were from two donors. Error Bars represent the 95% CI on the mean DNA yield (ug) per mg of tissue from replicate specimens (1-10 mg).
TABLE 1: The increase in yield for the Ionic Tissue to Pure DNA Kit vs. other kits is consistent across tissue types. The fold-change was calculated by dividing the mean DNA yield per mg of tissue for the Ionic kit by the mean DNA yield per mg of tissue for the respective comparison kit.
FIGURE 2: DNA yields were normalized to the Ionic kit yield for 1 mg on a by-tissue basis. The dotted line represents a theoretical linear extraction efficiency up to 10 mg tissue (assuming optimal recovery by Ionic kit at 1 mg specimens). The Ionic kit tracked the theoretical linear extraction efficiency more closely than the column-based methods.
TABLE 2: Each method was evaluated against common QC thresholds. The TapeStation Genomic DNA ScreenTape manual specifies a minimum DNA concentration of 5 ng/µL is required for accurate DIN score assignment. A higher percentage of eluates from the column-based approaches failed to meet the 5 ng/µL threshold. Ionic kit extracts also had lower observed DIN dropouts (no DIN score assigned) than the column-based approaches. Additionally, a higher percentage of Kit B eluates failed to meet the minimum recommended concentration for AmpliSeq Focus Panel library preparation compared to the Ionic kit.
Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip which applies an electric field to isolate nucleic acid in its natural, native form.
