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Purigen Biosystems, Inc.

Ionic® Purification Kits

Fluidic chips and reagents for the Ionic® Purification System

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Ionic Kits by Application

Ionic Purification Kits and protocols support automated purification of specific sample types and are required for use with the Ionic Purification System. Purification protocols are developed and validated at Purigen Biosystems to maximize purification yield and quality with minimal manual steps.

Ionic® Tissue to Pure DNA Kit
Ionic Cells to Pure DNA Kit

Each Tissue to Pure DNA Kit contains 6 Ionic Fluidic Chips and reagents to purify DNA from 48 samples.


Sample

Sample type Fresh frozen tissue
Sample preps per kit 48
Sample preps per chip 8

Input Amount

Tissue mass Up to 10 mg

Volume

Input volume per sample prep ~200 µL of lysed tissue
Extraction volume per sample prep ~50 µL

Run-time

Run-time per chip ~90 mins

Contents

Fluidic Chip 6 qty
Lysis Buffer 1 6 bottles; 1.6 mL
RNAse A 1 bottle; 249.0 µL
Proteinase K Reagent 1 bottle; 480.0 µL
Tissue Sample Buffer 1 bottle; 1.6 mL
Lysis Buffer 2 1 bottle; 4.0 mL
Extraction Buffer 1 bottle; 12.0 mL
Anodic Buffer 1 bottle; 12.0 mL
Separation Buffer 1 bottle; 18.0 mL
Neutralization Buffer 1 bottle; 12.0 mL
Cathodic Buffer 1 bottle; 12.0 mL

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Ionic® FFPE to Pure DNA Kit
Ionic FFPE to Pure DNA Kit

Each FFPE to Pure DNA Kit contains 6 Ionic Fluidic Chips and reagents to purify DNA from 48 samples.


Sample

Sample type FFPE scroll / tissue section
Sample preps per kit 48
Sample preps per chip 8

Cross-section

Maximum number of 10 µm thick scrolls/curls 3
Recommended cross-sectional area per scroll/curl 50 mm2 – 300 mm2

Volume

Input volume per sample prep ~200 µL of lysate
Elution volume per sample prep ~40 µL

Run-time

Run-time per chip ~60 mins

Contents

Fluidic Chip 6 qty
Lysis Buffer 6 bottles; 1.8 mL
RNAse Reagent 1 bottle; 250.0 µL
Proteinase K Reagent 1 bottle; 480 µL
Extraction Buffer 1 bottle; 12.0 mL
Anodic Buffer 1 bottle; 12.0 mL
Separation Buffer 6 bottles; 2.5 mL
Neutralization Buffer 1 bottle; 12.0 mL
Cathodic Buffer 1 bottle; 12.0 mL
Mineral Oil 1 bottle; 15.0 mL

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Ionic® FFPE to Pure RNA Kit
Ionic FFPE to Pure RNA Kit

Each FFPE to Pure RNA Kit contains 6 Ionic Fluidic Chips and reagents to purify RNA from 48 samples.


Sample

Sample type FFPE scroll / tissue section
Sample preps per kit 48
Sample preps per chip 8

Cross-section

Maximum number of 10 µm thick scrolls/curls 2
Recommended cross-sectional area per scroll/curl 50 mm2 – 300 mm2

Volume

Input volume per sample prep ~200 µL of lysate
Elution volume per sample prep ~40 µL

Run-time

Run-time per chip ~60 mins

Contents

Fluidic Chip 6 qty
Lysis Buffer 1 6 bottles; 1.4 mL
Lysis Buffer 2 1 bottle; 4.0 mL
DNAse I Reagent 1 bottle; 120.0 µL
Proteinase K Reagent 1 bottle; 1.0 mL
Extraction Buffer 1 bottle; 12.0 mL
Anodic Buffer 1 bottle; 12.0 mL
Separation Buffer 6 bottles; 2.5 mL
Neutralization Buffer 1 bottle; 12.0 mL
Cathodic Buffer 1 bottle; 12.0 mL
Mineral Oil 1 bottle; 20.0 mL

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Ionic® FFPE Complete Kit
Ionic FFPE to Pure RNA Kit

Each FFPE Complete Kit contains 12 Ionic Fluidic Chips and reagents to purify RNA and DNA from 48 samples.


Sample

Sample type FFPE scroll / tissue section
Sample preps per kit 48 (RNA and DNA from each)
Sample preps per chip 8

Cross-section

Maximum number of 10 µm thick scrolls/curls 2
Recommended cross-sectional area per scroll/curl 50 mm2 – 300 mm2

Volume

Input volume per sample prep ~200 µL of lysate
Extract volume per sample prep ~40 µL

Run-time

Run-time per chip ~60 mins

Contents

Fluidic Chip 12 qty
Lysis Buffer 1 12 bottles; 1.4 mL
Lysis Buffer 2 1 bottle; 7.0 mL
DNase I Reagent 1 bottle; 120.0 µL
RNase Reagent 1 bottle; 120.0 µL
Proteinase K Reagent 1 bottle; 1.5 mL
Extraction Buffer 1 bottle; 24.0 mL
Anodic Buffer 1 bottle; 24.0 mL
Separation Buffer 2 bottles; 15 mL
Neutralization Buffer 1 bottle; 24.0 mL
Cathodic Buffer 1 bottle; 24.0 mL
Mineral Oil 1 bottle; 20.0 mL

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Ionic Reagents and Kit

 

Purigen Isotachophoresis Technology

Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip which applies an electric field to isolate nucleic acid in its natural, native form.