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Purigen Biosystems, Inc.

A Superior Automated Approach to DNA and RNA Extraction

The Ionic Purification System is so different, its advantages are most readily understood in contrast with conventional DNA and RNA extraction and purification methods.


  • No organic solvents
  • No harsh, high-salt buffers
  • No system programming
  • No beads or repetitive washing
  • No hands-on mixing, separation, sample transfers, or buffer exchanges
  • No pumps, valves, or other moving parts

Ionic Purification System

The Ionic Purification System uses isotachophoresis (ITP)
to extract, purify, and concentrate genomic DNA and RNA
from cells and FFPE. DNA and RNA samples prepared
by the system are pure, abundant, and ready in just 60 minutes.

 

Easy to Use and Small Footprint

Pure DNA and RNA in Just ONE Hour

Purigen’s simplified workflow minimizes opportunities for material loss and errors. In a single automated step, the Ionic system yields pure, high-concentration DNA and RNA in a low-salt buffer.

  1. 1 Load Run Buffers

    Add buffers from reagent kit –
    use touchscreen to start priming

    8 mins

  2. 2 Load Sample

    Load 8 samples –
    use touchscreen to start the run

    50 mins

  3. 3 Get Purified Nucleic Acid

    Collect samples when touchscreen displays
    “Run Complete”

    2 mins

Purification in just 3 minutes of hands-on time per sample

Want Higher Amplifiable Yields?

Extract Up to 50% More Nucleic Acid from Cells and FFPE

 

qPCR results from the amplification of a 280bp amplicon in DNA purified from cell populations ranging from 1,120 to 56,000 cells demonstrate that purification on the Ionic system results in values that are ~3 Ct lower than those from a column-based method. For populations with fewer cells, some eluates from the column-based competitor do not contain detectable amounts of DNA.

 

Achieve Yields of 3.5x or More from FFPE Samples

Purigen SCROLLS vs. Column-based Kit SLIDES

 

Results for 32 FFPE block samples* purified from scrolls for Purigen (no paraffin removal) vs. slides (excess paraffin removed) for a column-based approach. Yields for Purigen samples demonstrate improved amplifiable yields of on average 3.5x versus column-based approach.

 


Want Higher Quality Results?

More Uniform Coverage

 

NGS results of DNA purified from FFPE samples analyzed using the Agilent SureMASTR Tumor Hotspot sequencing panel with 252 amplicons ranging from 128 bps to 245 bps show that samples purified on the Purigen Ionic system have tighter clustering when compared to samples prepared using a column-based method – indicative of more uniform sequence coverage.

 

No Bias Toward Length or GC Content of Amplicons

 

 

Taking a closer look at the results from above shows that DNA from FFPE samples purified on the Ionic system is less biased toward GC content and fragment length than from FFPE samples purified using a column-based method.

 

*Data for the graphs above is normalized by a reference sample to remove the effect of coverage differences introduced by the amplification efficiency of the target amplicon. Data from the reference sample incorporates purification results from the Purigen system and a column-based method to avoid biasing the reference sample toward either technology.

 

System Specifications

Dimensions and Weight

Height42.4 cm / 16.7 in
Width41.9 cm / 16.5 in
Depth48.9 cm / 19.3 in
Weight24.5 kg / 54 lb

Facility Requirements

North America / UL120 VAC, 60Hz, 2.5A
Europe / CE230 V~50 Hz, 1.0A
Power consumption300 watts

Regulatory Compliance

North America / UL• 61010
• CFR title 47, sub part B of part 15 / ICES-003, Issue 6
Europe / CE• EN61010-1:2010
• EN 61326-1-2013
• WEEE Directive 76/769/EC

 

Purigen Isotachophoresis Technology

Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip which applies an electric field to isolate nucleic acid in its natural, native form.