In collaboration with UCSF and Miroculus @ AGBT 2021

The Ionic Purification System for FFPE Samples

Simultaneous Extraction and Purification of RNA and DNA from FFPE Samples

The Ionic® Purification System has demonstrated advantages over traditional solid phase (column or bead) methods when it comes to extraction of RNA and DNA from Formalin-Fixed Paraffin Embedded (FFPE) tissue. When extracting nucleic acids from FFPE, the Ionic system routinely delivers 2-5 fold increases in yield as well as lower length and GC biases.

Macrodissection of FFPE tissue prior to nucleic acid purification allows the operator to select tissue of interest (typically tumor tissue) and minimize background tissue. This in turn can enrich the extracted nucleic acid for variants of interest. The Ionic Purification System from Purigen Biosystems is an excellent choice for macrodissected tissue. It offers a simple, automated work-flow and typically generates yields that are 2-4x higher than other methods, maximizing the amount of nucleic acids extracted from macrodissected tissue.

With a simplified workflow and separation based solely on the electrophoretic mobility of the molecules in a sample, Purigen’s Ionic Purification System offers an alternative for FFPE sample purification for NGS that has less risk of further damage to nucleic acids or bias toward specific fragment length or GC content.

FFPE tissue samples are known to produce low yields of nucleic acid that is often of a lower quality by comparison to nucleic acid from fresh frozen tissue, particularly samples that have been stored for several years or longer. When FFPE tissue is limited, conventional extraction and purification technologies may not produce enough high quality DNA to meet the recommended library preparation input requirements for next-generation sequencing (NGS).

Extract higher yields of nucleic acid from a single FFPE tissue section or as few as 10 cells.

Simultaneous extraction and purification of both RNA and DNA from FFPE tissue samples is critical to enable multiple downstream analyses from rare or limited samples. To avoid yield loss, most technologies use a serial isolation approach where DNA and RNA are separated after lysis by filtration or precipitation before entering separate purification workflows. This results in twice the number of purification steps per sample.